Sustainability in Plant and Crop Protection The use of phytoplasma-resistant host plants and of phytoplasma-free material for new plantings could represent a starting point for phytoplasma disease management. most of the production that was lost in the early stages of the watercress yellows epidemic on Oahu, and has prevented the spread of this pathogen to other islands in Hawaii.Publications, Progress 10/01/03 to 09/30/04OutputsThe incidence of yellows disease caused by phytoplasma on watercress farms on Oahu has decreased markedly over the last year, due to continued efforts by farmers to rogue out infected, symptomatic plants, and to control the population of the insect vector, Macrosteles sp., in their fields. Not logged in to achieve an effective disease control, with the lowest environmental impact, it is necessary to elucidate the FD strain once the phytoplasma is detected in a new or in infected areas after some years from an epidemic areas outbreak. Our analyses of these genes has revealed that the watercress phytoplasma is probably most closely related to the Aster Yellows-type phytoplasma that testing the suitability of selected vegetable and native Hawaiian plants as food sources and oviposition hosts for the insect. Part of Springer Nature. We are still conducting experiments using laboratory-reared Macrosteles sp. (ISBN: 9781402085703) from Amazon's Book Store. This information is helpful to reduce and localize the pesticide application against the insect vector All of the watercress farmers on Oahu are now aware of the problem this disease presents and of the most efficient ways to protect their farms from this threat., Kevin Muirhead, Edel Pérez-López, Brian W. Bahder, Janet E. Hill, Tim J. Dumonceaux, Mohammad Djavaheri, Maryam Ansari, M. Hossein Borhan, Majid Siampour, Keramatollah Izadpanah, Mohammad Salehi, Alireza Afsharifar, Karel Acosta, Madelaine Quiñones Pantoja, Edel Pérez-López, George O. Asudi, Francis N. Muyekho, Charles A. O. Midega, Zeyaur R. Khan, Philippe Giordanengo, Sébastien Boquel, Julien Saguez, Charles Vincent, J. Bila, A. Mondjana, B. Samils, L. Santos, N. Hogberg, Ivan Paulo Bedendo, João Roberto Spotti Lopes. This book will focus on detection and prevention of phytoplasma diseases in field and horticultural crops grown in the tropical belt. We have tested a variety of PCR primer-pairs for their ability to detect the presence of the pathogen in a variety of plants and have determined the relative sensitivities of these primer-pairs for detection of low levels of infection in various plants. We now have established colonies of this vector on plant hosts other than watercress on which they are able to complete their lifecycle, and have begun using these colonies to conduct controlled transmission tests of the pathogen to With 160+ countries and islands, the tropical belt is the geographical region centered on the equator and limited by the tropics of Cancer and Capricorn. The observation of the disease progress on these farms has provided us with the ability to formulate options for controlling the spread of this disease in watercress. We are in the process of testing selected host plants for their ability to be infected by the phytoplasma under controlled conditions in the laboratory.ImpactsThe identification of the causal agent of watercress yellows has been the major step in devising control strategies to limit the spread of this disease on watercress farms in Hawaii. This book will focus on detection and prevention of phytoplasma diseases in field and horticultural crops grown in the tropical belt. The recommendations that we have provided watercress farmers have allowed them to begin to reclaim the production levels that they were achieving before the introduction of this pathogen and its insect vector into the state. Sequence analysis of both the Rp and Tuf genes has been used to further classify phytoplasmas of the Aster Yellows group throughout the world. We have completed work on the host range of the insect vector, Macrosteles sp., testing the suitability of selected vegetable and native Hawaiian plants as food sources and oviposition hosts for the insect. causes Onion Yellows in Japan, and is less closely related to the phytoplasma that causes Severe Aster Yellows in western North America. Further characterization of the phytoplasma infecting watercress in Hawaii has included the cloning and sequencing of the Rp and Tuf genes in addition to the cloning and sequencing of the 16S/SR/23S ribosomal DNA genes previously characterized. This starts with good weed removal practices and clearing brush that can host insect vectors. This service is more advanced with JavaScript available, Part of the We will work with the growers and other researchers to examine and implement these control strategies based on an integrated pest management approach. The insect colonies that we have established will enable us to address specific questions regarding the details of pathogen transmission to various crops by this insect.Publications, Progress 10/01/02 to 09/30/03OutputsThe phytoplasma that causes a new disease of watercress has been identified and the incidence of disease on several large farms on Oahu has been monitored during this period.

management of phytoplasma diseases

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